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Magnetic Resonance studies and clinic evidence of the effect of Human Recombinant Erythropoietin (RHEPO) antigen induced arthiritis treatment


Rheumatoid arthritis (RA) is an autoimmune pathology where a high level of pro-inflammatory cytokines, like IL-1 and TNF, are produced at the sinovia, changing levels of nitric oxide (ON). Preliminary results showed that rhEPO treatment to rabbits with antigen induced arthritis (AIA) affected the progression of the pathology. Our aim was to evaluate the potential effect of these treatments. We studied the knees of 3 month-old female New Zealand rabbits: control (C) and AIA, both treated with rhEPO or not (p: placebo doses) during 30 days (n = 4 per group; 1000 U rhEPO ⁎kg-1 ⁎ 24 h-1). ON in serum was analyzed with the Griess method and by magnetic resonance (RM) to anaesthetized rabbits with small bones software:, with the following sequence: Inversion Recovery TR 2000, TE 60, NEX 3, 6 min acquisition time. No differences were detected among C-EPO and Cp treatments. Arthritic groups had higher transverse diameters: AIAp> AIA-EPO> C (p < 0.01). AIAp had level III to V bone h yperintensities with slight alterations of the soft parts (+to+). AIA-EPO showed level II to III bone hyperintensities, with very slight alterations of the soft parts (+). NO differences were detected: (AIAp>AIA-EPO>C (p < 0.01). The synovium of the AIAp animals were characterised by higher hyperplasia of the synovial lining cells and infiltration by lymphocyte, and plasmocytes than AIA-EPo(p < 0.01). Our results stress the need to carry out further studies on the action mechanisms of this pleiotropic hormone and of emergent therapies which promote the re-establishment of internal balance of affected individuals and maybe avoiding the use of chronic treatments with undesired side effects, such as corticoids


Bone-arthricular Biology and Emergent Therapies Lab., Cs Méd Universidad Nacional Rosario